Presentation Details
| Genetic engineering of megakaryocytes to uncover functions of platelet proteins Jesse Rowley. |
Abstract
As the precursor to platelets, megakaryocytes share many functions with platelets and are the natural choice for platelet function studies, but have been traditionally difficult to genetically modify. We have developed a CRISPR/CAS9 approach to generate and test gene deletions and precise genetic edits with high efficiency in human CD34+ cell derived megakaryocytes. We demonstrate both single and multiplex approaches to define proteins implicated in platelet function, and interrogate single nucleotide changes associated with inherited platelet disorders.
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