Presentation Details
| Comparative Protamine Sulfate and HEPA-Remove Neutralization Studies in Potency Adjusted Porcine and Bovine Heparin Emily Krupa, Debra Hoppensteadt-Moorman, Walter Jeske, Jawed Fareed. Loyola University Medical Center, Maywood, IL, USA |
Abstract
Background: Protamine sulfate acts as a heparin antagonist to reverse the anticoagulant effects of heparin through an acid-base reaction. Protamine is commonly used in clinical settings to neutralize heparin-induced anticoagulation in patients following surgery and other bleeding complications. HEPA-Remove is a novel heparin neutralizer thought to exhibit effects similar to protamine. Aim: The purpose of this study was to compare the neutralization activities of protamine sulfate and HEPA-Remove inn various laboratory assays when supplemented in potency adjusted bovine and porcine heparin samples. Methods: Bovine and porcine heparins samples were adjusted according to their USP potencies. The samples were supplemented in blook bank plasma (BBP) in a concentration range of 0-1 U/mL. Protamine sulfate and HEPA-Remove were freshly prepared and added to each heparin sample at a final concentration of 10 g/mL. Samples were then analyzed in an ACL Top 350 coagulation analyzer to determine their activated partial thromboplastin time (aPTT). Anti-Xa and anti-IIa assays were then conducted by amidolytic methods. Thrombin generation inhibition assays (TGA) studies were also done. Studies were repeated twice, and then statistical analysis was performed using SigmaPlot two-way ANOVA. Results: Table 1. Compiled average results and standard deviation in each assay and sample. Conclusion: Bovine and porcine heparin samples were similarly neutralized by protamine sulfate and HEPA-Remove in aPTT, anti-Xa, and anti-IIa studies, with some exceptions at higher concentrations.
No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, including photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author.
No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, including photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author.